Decitabine treatment of ALL cell lines shows reexpression of hypermethylated genes at relapse. (A) Real-time PCR was performed on Reh, UOCB1 (B-lineage), and Raji (lymphoma) cells treated with decitabine at a 1μM concentration for 48 hours (Reh and Raji) or 72 hours (UOCB1). Results are expressed relative to the levels observed at baseline without decitabine exposure. Each experiment was performed in triplicate. Error bars represent SD. (B) Determination of concordant changes in promoter methylation levels by MSP in Reh and UOCB1 cell lines. *CDKN2A mRNA expression levels and MSP were performed in Raji cells only.