Histone modifications at the promoters of genes of interest. (A) Western blot analysis of acetyl-H3 (and actin loading control) in Reh cells with and without vorinostat exposure. (B) ChIP was carried out with Abs specific for key H3 modifications associated with transcriptional activation (K9ac and K4me2/3) and transcriptional repression (K9me3 and K27me3) and purified DNA was subjected to RT-PCR using primer sets designed to amplify the transcriptional start site and/or the 5′ region of NR3C1, HRK, BIRC5, and FOXM1. Each graph shows the modification of these marks after vorinostat exposure normalized to log input (1%) and the corresponding mRNA expression of the gene by quantitative RT-PCR. TSS indicates transcriptional start site.