Kindlin-3 is not required for SFK-dependent TCR-mediated signaling and spreading. (A) Control (black bars) or Kindlin-3–null (white bars) PB T cells were pretreated with carrier (DMSO 0.1%) or PP2 (10μM for 30 minutes at 37°C) and allowed to spread on immobilized anti-CD3 mAb (OKT3, 20 μg/mL). Results are the mean ± range of 2 fields. The experiment shown is representative of 2. (B) PB T cells spread on anti-CD3 mAb as in panel A were fixed and stained with anti–phospho LAT antibodies. DIC and fluorescence images of cells are shown in a representative field. Scale bar represents 3 μm.