Bcl2-A1 is required for pDC differentiation in vitro. CD34⁺CD38⁻ HPCs from human FL were transduced with vectors expressing Bcl2-A1 shRNAs or renilla control shRNAs, and cocultured with OP9 stromal cells with IL-7 and Flt3L for 7 days. Flow cytometric analysis was performed to identify BDCA²⁺CD123⁺GFP⁺ in vitro generated pDCs or GFP⁺BDCA-2⁻ non-pDCs. Shown are the absolute cell numbers normalized to the control culture of 15 donors (*P = .012, ***P = .003). Calculations were done as described in “Methods.”