Increased bleeding time and failure of platelets to respond to VWF in gp96/grp94 KO mice. Bleeding time analysis of WT (□, n = 5) and KO (■, n = 5) mice (A) and WT → WT (□, n = 8) and KO → WT (■, n = 9) mice (B) 14 days PTD. *P < .05. (C) Aggregation of pooled purified WT (n = 10) and KO (n = 13) platelets 2 months PTD in response to VWF/botrocetin. (D) Representative adherence to immobilized VWF under shear stress of fluorescently labeled platelets from WT (n = 10) and KO (n = 13) mice 2 months PTD. (E) Average number of adhered platelets of WT and KO origin shown in panel D in 10 randomly selected fields. *P < .01.