D6^−/− mice are partially protected from fully mismatched GVHD. (A) Survival curves and (B) clinical scores of WT (□) or D6^−/− (■) B6 mice treated with BM and WS cells from BALB/c mice after myeloablation. Control mice (▾) are B6 recipients treated with syngeneic BM and WS. Data are representative of 2 independent experiments (n = 8 mice/group). (C-D) Absolute number of CD8⁺ and CD4⁺ cells in gut and liver of mice killed at day 8 and day 16 after transplantation in control group (gray bar), WT (white bar), and D6^−/− (black bar) B6 recipients. Concentration of mIFN-γ (E) and mCCL2/JE (F) in serum, liver, and gut of control (▾), WT (□), and D6^−/− (■) B6 recipients. Data are mean ± SD of 2 independent experiments (n = 10 mice/group). *P < .05, D6^−/− or WT mice versus control mice. #P < .05, D6^−/− versus WT mice.