Reduced T-cell proliferation in D6^−/− mice. (A) WS, Gr1⁺-depleted WS and (B) LNs taken from B6 D6^−/− (black bar) versus WT (white bar) mice were used as responders in a 1-way mixed leukocyte reaction. Graphs represent the total [³H]-thymidine uptake in the presence of BALB/c DCs as allogeneic stimulators. Data are mean ± SD and are representative of 3 independent experiments. *P < .05. **P < .005. (C) Absolute number ± SD of CD8⁺ OT-I in draining LN of D6^−/− (black bar) and WT (white bar) mice immunized with DEC205-OVA plus lipopolysaccharide treated with anti-Gr1 or isotypic antibody (200 μg) at the time of transfer and at day 1 after immunization. (D) CFSE dilution profile of transferred OT-I cells in LN of D6^−/− and WT mice for the indicated treatment (isotype or anti-Gr1). (E) Percentage of proliferating OT-I cells in LN of D6^−/− (black bar) and WT (white bar) mice. Data are representative of 2 independent experiments (n = 4 mice/group). *P < .05. **P < .005.