Increased accumulation of myeloid cells in the spleen of D6^−/− mice after CFA injection. FACS analysis of BM, spleen, and blood harvested from WT and D6^−/− mice injected subcutaneously with 200 μL of CFA in the right flank. BM CD11b⁺/Ly6C^high monocytes (A) and CD11b⁺/Ly6G⁺ granulocytes (D), expressed as fold increase of the percentage of CD45⁺ leukocytes, recovered at indicated time points. Fold increase of the absolute number of monocytes (B-C) and granulocytes (E-F) in blood and spleen of D6^−/− (■) versus WT (□) mice. (G) Fold increase of the absolute number of CD11b⁺/Ly6C^high monocytes in blood of D6^−/− (■) versus WT (□) mice after 4 weeks of splenectomy or sham operated before and after 48 hours CFA treatment. In all panels, data are representative of 2 independent experiments (n = 4 mice/time point). Data are mean ± SD. *P < .05 comparing D6^−/− and WT animals.