Preferential induction of Ly49G2 transcripts by HSCT and IL-2. RT-PCR (35 cycles) of Ly49 Pro1 transcripts was performed with primers specific for Ly49G2, Ly49a, and Ly49c/i. The identity of all products was confirmed by DNA sequencing. (A) Detection of Ly49 Pro1 transcripts after HSCT. RNA was isolated from the spleen, BM, or liver of mice receiving hematopoietic stem cell transfer HSCT or control mice. The numbers under each panel (Δ+HCST) indicate the average -fold induction of the transcript by HCST as measured by SYBR-Green quantitative PCR. (B) Induction of Ly49 Pro1 transcripts by in vivo IL-2 treatment. Induction (fold) in response to IL-2 treatment is shown under each panel (Δ+IL-2). Data are representative of 3 experiments.