Ly49G2 depletion results in deficient NK-cell numbers after congenic HSCT. Lethally irradiated B6 (H-2^b, CD45.2) mice were transplanted with 5 × 10⁶ Ly5.2 congenic (H-2^b, CD45.1) BMCs depleted of NK and T cells. A group of mice received purified anti-Ly49G2 (4D11) depleting Ab. Splenocytes were stained for CD45.1, CD122, Ly49C/I, Ly49G2, and Ly49A at days 14, 21, and 28 after congenic HSCT and compared with transplanted control mice. (A) Frequencies of NK-cell subsets. The numbers represent the percentages of cells in each quadrant. (B) Numbers of developing CD45.1⁺CD122⁺CD3⁻ cells. *P < .001; **P < .0001; ***P < .01. NS indicates not significant. Data are representative of 2 experiments using 3-4 mice per group per time point in each experiment (means ± SEM).