Long-term course of Ly49C/I and Ly49G2 expression on developing NK cells in CD45-congenic HSCT. Lethally irradiated B6 (H-2^b, CD45.2) mice were transplanted with 5 × 10⁶ Ly5.2 congenic (H-2^b, CD45.1) BMCs depleted of NK and T cells. Splenocytes of B6 (H-2^b) recipients were stained for CD45.1, NK1.1, CD3, Ly49C/I, and Ly49G2 at different time points after congenic HSCT and compared with untreated control mice. Gated CD45.1⁺NK1.1⁺CD3⁻ cells are shown. (A) Frequencies of NK-cell subsets. The numbers represent the percentages of cells in each quadrant. (B) Numbers of donor-derived NK cells after HSCT. *P < .001; **P < .01. NS indicates not significant. Data are representative of 3 experiments using 3-4 mice per group per time point in each experiment (means ± SEM).