In vivo binding of PU.1, NF-κB p65, and IRF4 to the CD80 or CD86 promoter in BMDCs. Quantitative analysis of PU.1 (A), NF-κB p65 (B), and IRF4 (C) binding to the CD80 or CD86 promoter region was performed by ChIP assay using real-time PCR. The results are expressed as means + SD for 3 PCRs with duplicates using murine BMDCs treated with IFNγ (100 ng/mL) for 24 hours and/or following stimulation by LPS (1 μg/mL) for 1 hour or without treatment. Closed bars, specific antibody; open bars, control antibody. Similar results were obtained in 2 additional experiments.