Adoptive transfer of PMNs and recruitment to S aureus–infected wounds. (A) FACS sorting of mature EGFP-PMNs (c-kit⁻EGFP^highGr-1^highCD11b^high cells) and cytospin image. To avoid any inclusion of cells with proliferative capacity, c-kit⁺-progenitor cells were gated out. The FACS-sorted cells were intravenously transferred (5 × 10⁶ cells in 0.1 mL of sterile saline) to C57BL/6 mice whose wounds were treated with either sterile saline or S aureus (1 × 10⁷ CFUs), and the kinetics of EGFP-PMNs within the wound were determined (B). (C) The half-life of wound EGFP-PMNs was quantified from EGFP-PMN kinetic data using the equations described in the supplemental Methods. Data are derived from 4 mice in each group and are expressed as means ± SEM. *P < .01 vs saline control group.