Effects of time-dependent immunodepletion of systemic PMNs on S aureus wound infection. (A) Experimental design for early and delayed depletion of systemic EGFP-PMNs. Systemic PMNs were depleted with anti-neutrophil antibody treatment beginning either 24 hours before (pre-Gr-1) or 24 hours after (post–Gr-1) the onset of the infection (1 × 10⁷ CFUs of S aureus) and then every 2 days up to day 5. Shown are the kinetics of wound EGFP-PMNs (B), S aureus burden (C), and wound closure (D). Data are derived from 5-6 mice in each group and are expressed as means ± SEM. *P < .05 vs SA-isotype control group.