Effects of S aureus inoculum on PMN trafficking and tissue healing. EGFP-lys-mice were inoculated with saline or low-dose S aureus (2 × 10⁶ CFUs, SA-2e6 CFUs), or sublethal high dose S aureus (1 × 10⁷ CFUs, SA-1e7 CFUs) on back skin wounds, and kinetics of wound EGFP-PMN fluorescence (A-B), S aureus (SA) bioluminescence (C-D), and wound closure (E-F) were determined. Depicted are representative images of wound EGFP-PMNs (B), bioluminescent S aureus (D), and wound size (F). Data are derived from 6-8 mice in each group and are expressed as means ± SEM. *P < .05 vs saline group; #P < .05 vs SA-2e6 CFU group.