Figure 6
MHC class II contributed to the proliferation induced by the interaction between purified leukemic cells and monocytes. (A) Purified leukemic cells and monocytes were cultured in the same chamber or a different chamber of the Transwell (0.4-μm) for 6 days. 3H-thymidine was added to the culture during the last 6 hours of the culture. (B) Proliferation of purified leukemic cells cultured with or without 10 ng/mL recombinant human IL-1-α, IL-1b, IL-6, IL-12, IL-2, and IL-9. (C) Six-day spontaneous proliferation of purified leukemic cells, monocytes, and mixtures of purified leukemic cells and monocytes (leukemic cells: monocytes = 1:1). 3H-thymidine was added to the culture during the last 6 hours of culture. Anti-MHC class II or control antibody UPC10 were added to the culture at day 0. (D) Six-day spontaneous proliferation of smoldering/chronic ATL PBMCs with or without anti-MHC class II antibody and control antibody UPC10. 3H-thymidine was added to the cultures during the last 6 hours of culture. Anti-MHC class II or control antibody UPC10 were added to the cultures at day 0. The data are representative of data from 4 different patients.

MHC class II contributed to the proliferation induced by the interaction between purified leukemic cells and monocytes. (A) Purified leukemic cells and monocytes were cultured in the same chamber or a different chamber of the Transwell (0.4-μm) for 6 days. 3H-thymidine was added to the culture during the last 6 hours of the culture. (B) Proliferation of purified leukemic cells cultured with or without 10 ng/mL recombinant human IL-1-α, IL-1b, IL-6, IL-12, IL-2, and IL-9. (C) Six-day spontaneous proliferation of purified leukemic cells, monocytes, and mixtures of purified leukemic cells and monocytes (leukemic cells: monocytes = 1:1). 3H-thymidine was added to the culture during the last 6 hours of culture. Anti-MHC class II or control antibody UPC10 were added to the culture at day 0. (D) Six-day spontaneous proliferation of smoldering/chronic ATL PBMCs with or without anti-MHC class II antibody and control antibody UPC10. 3H-thymidine was added to the cultures during the last 6 hours of culture. Anti-MHC class II or control antibody UPC10 were added to the cultures at day 0. The data are representative of data from 4 different patients.

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