Effect of topical imiquimod on LC frequency and phenotype in the skin epithelium. (A) Contour plots showing GFP reporter expression (specific for host LCs) in skin epithelia of Langerin.DTR/GFP mice treated 24 hours previously with topical imiquimod (Imq) or control. (B) Left: Contour plots showing dual staining for CD86 and MHC class II in gated LCs from mice treated 24 hours previously with topical imiquimod or control. Right: Summary data showing the percentage of CD11b⁺ LCs that were CD86^high MHCII^high, in control or imiquimod-treated mice (n = 4 or 5 mice per group); data pooled from 3 independent experiments. *P < .05. The mean is indicated by the horizontal bar.