The PHI DMOG and Dex have overlapping effects on gene expression in BFU-E cells. Next-generation mRNA sequencing was performed on mRNA extracted from BFU-E (CD71 and CD24a10%high) cells cultured 4 hours in SFELE medium with 100nM Dex, with 333mM DMOG; or with both 100nM Dex and 333mM DMOG. Expression of the 9636 most highly expressed genes (multiplied RPKM > 0 from the 4 groups) from cells treated with Dex, DMOG, and DEX + DMOG was compared with cells cultured in SFELE medium only. The left Venn diagram shows the overlap of the genes that increased > 50%, whereas the Venn diagram to the right shows overlap of genes that decreased to < 50% of that in unstimulated BFU-Es. Significance of the overlapping genes was computed with the use of hypergeometric distribution over all genes detected in any of the samples. The statistical significance of each respective overlap is presented below each diagram. The individual genes in each overlapping group are listed in supplemental Tables 5 and 6.
