A fraction of CMML mice acquire uniparental disomy of oncogenic Nras allele. (A) Western blot analysis of total Nras expression levels in bone marrow cells from representative animals. Note: Nras expression is completely absent in LSL/LSL mice due to the stop cassette, whereas Nras expression in LSL/+ mice is half of that of wild-type (WT) mice. (B) Total RNA and genomic DNA were extracted from bone marrow cells. Pyrosequencing of RT-PCR amplified products and direct sequencing of genomic PCR products at the G12 codon in representative animals are shown. The wild-type and mutated nucleotides are highlighted in yellow.