Phagocytosis induces a strong inflammatory response in splenic mononuclear phagocytes. (A) Heat-killed pHrodo bacteria were injected into the circulation of mhc2dab:GFP adults. Eighteen hours later, spleens were harvested and analyzed by cytometry. As shown on the FACS plot, 2% of the splenocytes phagocytosed bacteria, with all of them expressing the mhc2dab:GFP reporter. (B) Q-PCR expression for inflammatory genes (il-1beta and il-12p40) was performed on phagocytic and nonphagocytic control mhc2dab⁺ myeloid splenocytes. Whereas expression of csf1r was not changed after phagocytosis, the expression of il-1beta and il-12p40 was up-regulated. Units on the y-axis represent changes (fold) above whole splenocytes. Error bars indicate SD.