Visualization of DC-like cells in adult skin. (A) Skin from a cd45:DsRed, mhc2dab:GFP double-transgenic adult fish imaged with confocal microscopy. The GFP (left), DsRed (middle), and merged (right) images are shown. Three populations of cells could be isolated based on their expression of the 2 transgenes and their different morphologies. (B) Q-PCR expression for genes specific to macrophages and DCs (marco, csf1r), neutrophils (mpx), and B cells (igm) for sorted cells from the skin. Units on the y-axis represent changes (fold) above whole skin. Error bars indicate SD.