Isolation of a pure population of mononuclear phagocytes in WKM. (A) Distribution of cd45:DsRed and mhc2dab:GFP fluorescence in the myeloid gate (i), in the precursor gate (ii), and in a gate encompassing the myeloid and precursor gates (iii). cd45:DsRed^high; mhc2dab:GFP^high mononuclear phagocytes (red gate) were reanalyzed by forward and side scatter, showing that these cells overlap the myeloid and precursor fractions in WKM (iv). (B) Morphology of cd45:DsRed⁺; mhc2dab:GFP⁺ double-positive cells isolated from the myeloid fraction in WKM. Cells were cytospun and stained with May-Grünwald-Giemsa. The majority of the cells show the characteristics of macrophages, with kidney-shaped nuclei and vacuoles. Rare cells presented a different morphology with dendrites and a higher nuclear/cytoplasm ratio. The scale bar indicates 20 μm. (C) Q-PCR expression for genes specific to the neutrophil lineage (top panels) and the MPS (bottom panels). Whereas cd45:DsRed⁺; mhc2dab:GFP⁻ cells were highly enriched for neutrophil genes (top panels), cd45:DsRed⁺; mhc2dab:GFP⁺ cells expressed macrophage-specific genes only (bottom panels). Units on the y-axis represent changes (fold) above WKM. Error bars indicate SD.