Early T-cell development is restored in Miz-1^ΔPOZ cells by SOCS1 knockdown or Bcl-2 overexpression in vitro. (A) A total of 1000 sorted LSK cells from WT or Miz-1^ΔPOZ were incubated with morpholino against SOCS1 mRNA. Comparative in vitro differentiation kinetics of the cells was monitored after 6 to 20 days of coculture on OP9DL1. (B) A total of 500 sorted ETP cells from Bcl-2Tg or Miz-1^ΔPOZ x Bcl-2Tg were analyzed by FACS after 6 to 20 days of culture. Gated live cells were further gated on CD4⁻CD8⁻TCR-γδ⁻ and analyzed for CD44 and CD25 surface expression to assess DN stages of differentiation. CD4 and CD8 surface expression shows development progression of more mature T cells. Data are representative of 4 independent experiments for panel A and 5 experiments for panel B.