Miz-1^ΔPOZ DN thymocytes show aberrant IL-7-related gene expression profile. (A) RNA was extracted from 5 to 10 × 10³ sorted DN1 and DN2 cells. All values are normalized to the expression of GAPDH gene and are presented as fold induction relative to values obtained with the respective wild-type control (set as 1-fold). Average of triplicate values ± SD are shown (n = 5). (B-C) Quantitative real-time PCR analyses of indicated genes in sorted DN1 and DN2 cells nonactivated (NA) or activated with IL-7 for 4 hours. Data are presented as fold induction relative to values obtained with the respective NA sample for wild-type control (left panel) and for Miz-1^ΔPOZ (right panel). Average of triplicate values ± SD are shown (n = 3).