Figure 2
![Figure 2. Colocalization of PAI-1 and norepinephrine in sucrose density gradient fractions. PC12 cells were labeled with [3H]norepinephrine and fractionated on a continuous sucrose density gradient as described in “Sucrose gradient fractionation.” Fractions were collected and assayed for [3H]norepinephrine by liquid scintillation counting (squares), PAI-1 by ELISA (circles), and sucrose concentration by refractometry (triangles; A). Fractions 10 to 19 were further analyzed by Western blotting with anti–PAI-1 antibody as described in “Western blotting” (B).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/26/10.1182_blood-2010-05-287672/5/zh89991174100002.jpeg?Expires=1722706075&Signature=19TE4~NGtlsdpqJORUMU7T9xr2kK8C9ZzRbPJh5hYbLhtK2We6jGJxKKU4E9yjUz1~vfWWDBJwdbqwyMxCuhnMC3~aWcGXPXUg7pdAnJpPfh0EFJR5aeBGy1L37LU5Q3WkjUqmxG4GPifJTfLN1D~hDKDNYA6HFFlObLw9Krzm0qJjikgiC4jR9qipWs6G~Ao5J8KMmSX0LQSOpRkkjZIC7jDRhMJZ6Kk5065B~sOcfYOh2CEuRaTQsIZL-UeCBzeLiiH7h5Crosncrfz25olqqGdsfhvWRjNvlLjhBRU31gOb2etw3XkNSdVgW~GGlWYOz4wYGKUbDqQi1nDsjdvw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Colocalization of PAI-1 and norepinephrine in sucrose density gradient fractions. PC12 cells were labeled with [3H]norepinephrine and fractionated on a continuous sucrose density gradient as described in “Sucrose gradient fractionation.” Fractions were collected and assayed for [3H]norepinephrine by liquid scintillation counting (squares), PAI-1 by ELISA (circles), and sucrose concentration by refractometry (triangles; A). Fractions 10 to 19 were further analyzed by Western blotting with anti–PAI-1 antibody as described in “Western blotting” (B).
