Figure 1
![Figure 1. Expression of PAI-1 in catecholaminergic cells. Left Panel: Northern blot analysis of PAI-1 mRNA expression in rat PC12 pheochromocytoma cells. Twenty-five μg of total RNA from either untreated or NGF-treated PC12 cells were loaded and separated on a 1% formaldehyde agarose gel. [α-32P]-labeled PAI-1 cDNA and rat cyclophilin cDNA were used as specific and control probes, respectively. The positions of 28 S and 18 S ribosomal RNA are shown. Right Panel: Expression of PAI-1 antigen in PC12 cells (A) and bovine adrenal medulla (B). Expression of PAI-1 antigen was investigated by Western blot analyses using specific anti–PAI-1 antibodies, or control normal rabbit IgG.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/26/10.1182_blood-2010-05-287672/5/zh89991174100001.jpeg?Expires=1722705547&Signature=4bIUAYtaT37GmxFN4Y5IKav9Mk1Uq9XzpYgrLc6AX2nzIoRErbvndOx7YfK6ntBvMOfcDpj-XndCdnGjnIW60QFDMhU5aJe~cISPzA2i0ix3r3RDyibqs3DwdkcJmtchmBeaRhMWhiMoUqwjc29BLB5TEX2xIuF1GrRUAGQ61nFRU~FwyXbWtEedi5iS1AC4h~tUaHNFA7V1M24EfUXfwimqZlZSfMR5YrF~VXdq88LnvD6lhFYynDDc00OMUKuzPiliWbT42t56j9DtenYEsPkU6Xq2YVRbBfEC9sIBtWp~Sv8ePDH1KTcq~7sOZbCap5AMbx56IhX5lgSBCYq2wQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of PAI-1 in catecholaminergic cells. Left Panel: Northern blot analysis of PAI-1 mRNA expression in rat PC12 pheochromocytoma cells. Twenty-five μg of total RNA from either untreated or NGF-treated PC12 cells were loaded and separated on a 1% formaldehyde agarose gel. [α-32P]-labeled PAI-1 cDNA and rat cyclophilin cDNA were used as specific and control probes, respectively. The positions of 28 S and 18 S ribosomal RNA are shown. Right Panel: Expression of PAI-1 antigen in PC12 cells (A) and bovine adrenal medulla (B). Expression of PAI-1 antigen was investigated by Western blot analyses using specific anti–PAI-1 antibodies, or control normal rabbit IgG.
