Validation of array-based gene expression profiles. (A) Quantitative PCR. (B) Immunoblot. Expression of CD86, CXCL10, CD52, and MSC was determined in KM-H2 and L428 cells, expressing KLF4-ER or empty vector, treated and not treated with 200nM of 4-OHT. After 24 hours of incubation with 4-OHT, cells were harvested. mRNA expression was determined by quantitative PCR and calculated in relation to internal control gene RPL13A by the comparative C_t method. Mean value of the group expressing empty vector and not activated with 4-OHT was used as comparator. Data are mean ± SD.