Figure 2
Figure 2. Blood, BM, and spleen features from Jak2V617F KI mice. (A) Blood smears from a 31-week-old Jak2V617F KI mouse revealed RBC polychromatophilia, anisocytosis and poikilocytosis, large platelets, and erythroblastosis (May-Grünwald-Giemsa staining). Images were obtained (63× lens) using a Zeiss Axiophot microscope with a Zeiss AxioCam Mrc camera and AxioVision Rel.4.3 acquisition software. (B) Spleen weight in KI mice proportionally increased with age (mean value ± SD, n = 3). (C) Myeloid trilineage hyperplasia in KI mice. The left panel shows no blockade in the differentiation processes and increases in the cumulative numbers (BM plus spleen) of granulocytic and erythroid precursor cells in KI compared with WT mice (mean value ± SD, n = 2, 27 and 31 weeks old). Calculation is based on the assumption that 1 femur represented 6% of the total marrow and from the number of cells isolated from the spleen. Cell types were identified from May-Grünwald-Giemsa stained cell cytospins. BL indicates blast; MY, myeloblast/myelocyte; ME/PN, metamyelocyte/polymorph; LY, lymphocyte; MO, monocyte; EO, eosinophil; and ERY, erythroblast. The right panel shows clustered megakaryocytes (MKs) with multilobulated nucleus from a hematoxylin/eosin/safran (HES)–stained KI mouse spleen (400× magnification). Images were obtained using a DM2000 Leica microscope and a DFC300FX Leica camera with Leica Application Suite v.2.5,OR1 acquisition software. (D-E) Histology of the spleen. (D) Hematoxylin-eosin coloration of a 12-week-old KI mouse revealed hyperplasia of the red pulp with the partially preserved white pulp that was clearly visible in a WT mouse (arrows). In contrast, white pulp of a 27-week-old KI mouse was completely blended. (25× magnification). (E) Silver stain coloration indicated high-grade fibrosis in a 36-week-old mouse. Fibrosis was absent in 12-week-old KI and 27-week-old WT mice (200× magnification). (F) Cumulative numbers of progenitor cells in BM and spleen from KI and WT mice scored after 2 (CFU-E) or 7 (BFU-E and CFU-GM) days of culture (mean value ± SE, n = 4, 21 ± 5 weeks of age). WT littermates were usually used as controls.

Blood, BM, and spleen features from Jak2V617F KI mice. (A) Blood smears from a 31-week-old Jak2V617F KI mouse revealed RBC polychromatophilia, anisocytosis and poikilocytosis, large platelets, and erythroblastosis (May-Grünwald-Giemsa staining). Images were obtained (63× lens) using a Zeiss Axiophot microscope with a Zeiss AxioCam Mrc camera and AxioVision Rel.4.3 acquisition software. (B) Spleen weight in KI mice proportionally increased with age (mean value ± SD, n = 3). (C) Myeloid trilineage hyperplasia in KI mice. The left panel shows no blockade in the differentiation processes and increases in the cumulative numbers (BM plus spleen) of granulocytic and erythroid precursor cells in KI compared with WT mice (mean value ± SD, n = 2, 27 and 31 weeks old). Calculation is based on the assumption that 1 femur represented 6% of the total marrow and from the number of cells isolated from the spleen. Cell types were identified from May-Grünwald-Giemsa stained cell cytospins. BL indicates blast; MY, myeloblast/myelocyte; ME/PN, metamyelocyte/polymorph; LY, lymphocyte; MO, monocyte; EO, eosinophil; and ERY, erythroblast. The right panel shows clustered megakaryocytes (MKs) with multilobulated nucleus from a hematoxylin/eosin/safran (HES)–stained KI mouse spleen (400× magnification). Images were obtained using a DM2000 Leica microscope and a DFC300FX Leica camera with Leica Application Suite v.2.5,OR1 acquisition software. (D-E) Histology of the spleen. (D) Hematoxylin-eosin coloration of a 12-week-old KI mouse revealed hyperplasia of the red pulp with the partially preserved white pulp that was clearly visible in a WT mouse (arrows). In contrast, white pulp of a 27-week-old KI mouse was completely blended. (25× magnification). (E) Silver stain coloration indicated high-grade fibrosis in a 36-week-old mouse. Fibrosis was absent in 12-week-old KI and 27-week-old WT mice (200× magnification). (F) Cumulative numbers of progenitor cells in BM and spleen from KI and WT mice scored after 2 (CFU-E) or 7 (BFU-E and CFU-GM) days of culture (mean value ± SE, n = 4, 21 ± 5 weeks of age). WT littermates were usually used as controls.

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