Ischemic muscles recruit Gr1^dimCD11b⁺ cells after femoral artery dissection. Cell suspensions from nonsurgically treated muscles and ischemic muscles of femoral artery dissected C57BL/6 mice were stained with anti-CD11b and anti-Gr1 monoclonal antibodies. (A) Representative dot plots from nonsurgically treated muscles of C57BL/6 mice and ischemic muscles of femoral artery dissected C57BL/6. Percentages of cells are shown as the mean ± SEM (B) Total number of Gr1^dimCD11b⁺/g tissue (n = 5 each). (C) Flow cytometric analysis of Gr1^dimCD11b⁺ cells. Curves to right in each panel indicate staining with specific antibody, and curves to left represent staining with isolated control antibodies. (D) Real-time q-PCR expression profile of MCP-1, SDF-1, MIP-1α, and VEGF in ischemic muscle before surgery, and 4 and 10 days after surgery.