ZAP-70-GFP, ZAP-70(SH2)2-GFP and LFA-1–mediated migration. (A) Schematic of ZAP-70-GFP and ZAP-70(SH2)2-GFP proteins encoded by cDNA constructs used in this study. (B) HSB2 T cells transfected with either ZAP-70-GFP or ZAP-70(SH2)2-GFP cDNAs by electroporation and allowed after 24 hours to migrate on ICAM-1; representative of n = 2 experiments per treatment. (C) HSB2 T cells that were electroporated with either GFP, ZAP-70-GFP, or ZAP-70(SH2)2-GFP cDNA constructs were allowed to migrate on ICAM-1 for 20 minutes. A boxplot of 3 independent migration experiments is shown and in panel D, a representative experiment of the migratory tracks of individual cells of each transfectant; n = 40 cells per condition.