Phospho-ZAP-70 colocalizes with high-affinity LFA-1 and talin at the lamellar/lamellipodial interface of migrating T cells. Confocal images of typical T lymphoblasts fixed on ICAM-1 before mAb staining. Images were taken at substrate level and merged images are shown in the right panel. The cells were stained with Alexa 546–conjugated mAb 24 or with mAbs specific for phospho-ZAP-70 and talin which were detected with the Alexa 488 or Alexa 546–conjugated anti–mouse IgG: (A) phospho-ZAP-70 (Y319; green) and mAb 24 (red); (B) phospho-ZAP-70 (Y319; green) and talin (red); (C) talin (green) and mAb 24 (red).