MK migration on collagen matrix is impaired. Purified BM-derived mature MKs adherent on fibronectin (20 μg/mL) or collagen (100 μg/mL)-coated coverslip were allowed to migrate toward a SDF1α gradient over 3 hours within the Dunn chamber. (A) Representative differential interference contrast (DIC) images from 6 independent experiments of primary MKs exposed to SDF1α gradient are shown (scale bar = 20μm). (B) The migration paths over 3 hours were traced and the net translocation distance of each cell on fibronectin and collagen matrix are represented. ***P < .005. See also supplemental Figure 6 video 3.