Aurk inhibition triggers mitotic arrest and polyploidy and induces apoptosis of Eμ-Myc lymphoma cells irrespective of the p53 status. (A) Eμ-Myc lymphoma cells cultured ex vivo were treated for the indicated times with 25nM AS703569 (AKI) and assessed for DNA content by flow cytometric analysis of PI-stained cells. The PI-stained cells were analyzed using the FL2 channel in a linear scale. Shown are representative histograms. The quantification and statistical analyses of 3 independently performed experiments are provided in supplemental Figure 4. (B) Eμ-Myc lymphoma cells were treated for the indicated times with the AKI AS703569 (25nM). Expression of the indicated proteins was assessed using immunoblotting. (C) Untreated Eμ-Myc lymphoma cells or cells treated with 25nM AS703569 (AKI) for the indicated times were analyzed for their apoptotic index by staining with annexin V–PI. Cells in the top right quadrant represent late apoptotic/necrotic cells. Cells in the bottom right quadrant represent early apoptotic cells. The percentage of cells in these quadrants is given. Shown is one representative dot blot graph of 3 independently performed experiments.