Up-regulation of TF, FVII, PAR1, and PAR2 in glioma cell lines expressing EGFRvIII oncogene. (A) TF antigen expression on the surface of 2 different GBM cell lines (U373 and U87) and their sublines transfected with EGFRvIII (fluorescence-activated cell sorting). (B) TF procoagulant activity assay (TF PCA) indicating the increased ability of EGFRvIII-transfected GBM cells to generate factor Xa (calibrated to the standard rabbit brain thromboplastin). Control (%) indicates the value obtained for U373vIII cells, which was the most procoagulant cell line in this panel (U373 and U373vIII, **P < .005; and U87 and U87vIII, *P < .05; N = 4). (C) The impact of EGFRvIII on the simultaneous expression of TF, PAR1, PAR2, and FVII mRNA in 2 different GBM cell lines U373 and U87 (reverse-transcribed polymerase chain reaction; “Experimental procedures” and supplemental data).