Figure 4
Figure 4. apln- and apj-deficient mice have impaired retinal vessel outgrowth. IsolectinB4 (green) staining visualizes the impaired retinal vessel outgrowth in APLN+/− and APLN−/− mice compared with wild-type mice at P2 (A-C) and P7 (D-E). (F) Quantification of vessel outgrowth in wild-type, APJ-, and apln-deficient mice at P7. (G-H) Vessel growth into deeper layers at P12 is delayed in apln−/− mice compared with wild-type mice. (J-K) Delay in vessel outgrowth in apj-deficient mice. (I,L) Quantification of branchpoints in APLN−/− and APJ−/− retinas compared with wild-type littermates, respectively. ***P < .001. *P < .05. Mann-Whitney test was used. n = 6 mice per group. Error bars indicate SEM. Scale bars indicate 300 μm (A-C); 600 μm (D,E,G,H,J,K). Images of antibody stainings were acquired on a Leica TCS SP5 confocal microscope using a 10×/0.3 NA objective.

apln- and apj-deficient mice have impaired retinal vessel outgrowth. IsolectinB4 (green) staining visualizes the impaired retinal vessel outgrowth in APLN+/− and APLN−/− mice compared with wild-type mice at P2 (A-C) and P7 (D-E). (F) Quantification of vessel outgrowth in wild-type, APJ-, and apln-deficient mice at P7. (G-H) Vessel growth into deeper layers at P12 is delayed in apln−/− mice compared with wild-type mice. (J-K) Delay in vessel outgrowth in apj-deficient mice. (I,L) Quantification of branchpoints in APLN−/− and APJ−/− retinas compared with wild-type littermates, respectively. ***P < .001. *P < .05. Mann-Whitney test was used. n = 6 mice per group. Error bars indicate SEM. Scale bars indicate 300 μm (A-C); 600 μm (D,E,G,H,J,K). Images of antibody stainings were acquired on a Leica TCS SP5 confocal microscope using a 10×/0.3 NA objective.

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