SENEX regulates adhesion. (A) SENEX- and EV-infected cells were stimulated with TNFα (5 ng/mL) for 5 hours. Adhesion of neutrophils was then assessed (ii). This is a representative of 3 experiments. Arrows show senescent cells from the corresponding fluorescence view in subpanel i. Bar = 220 μm. (B) HUVECs were treated as in panel A. Adhesion of mononuclear cells was then assessed (ii). This is a representative of 3 experiments. Arrows show senescent cells from the corresponding fluorescence view (i). Bar = 220 μm. (C) From the photographs taken in panel A, counts were made of the number of adherent neutrophils on a senescent cell (■).The number of adherent neutrophils were then counted in the same surface area on neighbouring nonsenescent cells (□). This is a representative of 102 senescent cells and the corresponding area of nonsenescent cells from 3 HUVEC lines. ***P < .001 compared with EV. (D) From the photographs in panel B, counts were taken of the number of adherent mononuclear cells on a senescent cell (■). The number of adherent mononuclear cells was then counted in the same surface area on neighbouring nonsenescent cells (□). This is a representative of the mean ± SEM of 42 senescent cells from 3 HUVEC lines. ***P < .001 compared with EV.