SENEX regulates angiogenesis and is required for EC survival. (A) HUVECs were infected with EV (i) or SENEX anti sense (ii) containing adenovirus. After 24 hours cells were plated onto Matrigel, and capillary tube formation was observed over a 24-hour time course. Photographs taken 12 hours after plating are shown. This is a representative of 3 similar experiments performed. Bars = 500 μm. (B) Expression levels of SENEX protein in HUVECs at 24 and 48 hours after infection with EV (EV) or SENEX (S) in the sense orientation in adenovirus and detected by Western blot analysis. Protein molecular masses (in kDa) appear on the right. This is a representatative of 5 experiments. (C) HUVECs were infected with EV (i) or SENEX (ii) containing adenovirus. Infected cells are visualized with green fluorescent protein (GFP). Photographs were taken after 48 hours. Bar = 220 μm. (iii) Enlarged area of cells from (B). Bar = 80 μm. (D) Phase contrast photograph of several enlarged cells. Bar = 80 μm.