Akt phosphorylation and Rap1 activation in G_i2α(+/G184S) platelets. (A) Phosphorylation of Akt after addition of the PAR4 agonist peptide AYPGKF (250μM). Mean ± SEM of data from 3 experiments. One mouse of each genotype was used in each experiment. Samples from the representative pAkt blot were run on the same gel. (B) Rap1 activation in response to varying concentrations of ADP. Mean ± SEM of data from 3 experiments. Two mice of each genotype were used in each experiment. Samples from the representative Rap1 blot were run on the same gel.