miRNA-9*–dependent modulation of WM cell proliferation in the context of BM milieu. (A) BCWM.1 cells (untransfected, scramble probe–, pre-miRNA-9*–transfected) were cultured for 48 hours in the presence or absence of primary BM stromal cells (BMSCs). Cell proliferation was assessed with the use of the [³H]-thymidine uptake assay. (B) BCWM.1 cells (untransfected, scramble probe–, pre-miRNA-9*–transfected) were cultured in the presence or absence of IL-6 (25 ng/mL) or IGF-1 (50 ng/mL) for 48 hours. DNA synthesis was assessed with the use of the [³H]-thymidine uptake assay. (C) BCWM.1 cells were cultured with LBH589 (0-40nM) for 48 hours in the presence or absence of WM patient-derived BMSCs. Cell proliferation was assessed with the use of the [3H]-thymidine uptake assay. (D) BCWM.1 cells were cultured with LBH589 (0-40nM) in the absence and presence of IL-6 (25 ng/mL) or IGF-1 (50 ng/mL) for 48 hours. DNA synthesis was assessed with the use of the [3H]-thymidine uptake assay. All P ≤ .05.