Immunohistochemical analysis of TF expression. TF expression was analyzed in lungs of untreated TF^flox/flox mice (A) or TF^flox/flox (B) and TF^flox/flox,Tie-2^Cre (C) mice 8 hours after LPS injection. TF-positive cells are brown. ↑ indicates leukocytes and indicates ECs. Incubation of serial sections with control rat IgG demonstrated no staining (data not shown).