Figure 2
Figure 2. Analysis of TFflox/flox, LysMCre mice. (A) PCR analysis of mouse TF DNA isolate from blood cells demonstrates partial deletion of TF gene. Arrows indicate PCR products for wild type and floxed alleles. Note a nonspecific band below the TF band. The mouse interleukin-2 (IL-2) gene was used as a loading control. WT indicates DNA from a WT mouse. (B) Real-time PCR analysis of TF mRNA in blood cells isolated from TFflox/flox and TFflox/flox,LysMCre mice before (n = 2 per group) and after (n = 4 per group) LPS injection (2 hours). (C) Plasma TAT levels were analyzed in TFflox/flox (n = 35; mean ± SEM 19.4 ± 1.6 ng/mL) and TFflox/flox,LysMCre (n = 36; mean ± SEM 12.4 ± 1.4 ng/mL) mice 8 hours after LPS injection. *P < .01.

Analysis of TFflox/flox, LysMCre mice. (A) PCR analysis of mouse TF DNA isolate from blood cells demonstrates partial deletion of TF gene. Arrows indicate PCR products for wild type and floxed alleles. Note a nonspecific band below the TF band. The mouse interleukin-2 (IL-2) gene was used as a loading control. WT indicates DNA from a WT mouse. (B) Real-time PCR analysis of TF mRNA in blood cells isolated from TFflox/flox and TFflox/flox,LysMCre mice before (n = 2 per group) and after (n = 4 per group) LPS injection (2 hours). (C) Plasma TAT levels were analyzed in TFflox/flox (n = 35; mean ± SEM 19.4 ± 1.6 ng/mL) and TFflox/flox,LysMCre (n = 36; mean ± SEM 12.4 ± 1.4 ng/mL) mice 8 hours after LPS injection. *P < .01.

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