Targeting to hDEC205 enhances humoral responses. At day 0, hDEC205 Tg mice were primed by intraperitonal injection with 10 μg of purified 3G9, 3D6, or control human IgG1 mAbs in the presence of 50 μg of poly IC and 25 μg of anti-CD40 mAb. At day 25, mice were boosted intraperitoneally with 10 μg of the same antibodies without adjuvants. Serum samples were collected at days −2, 5, 21, 31, 40, and 58. Serum antihuman IgG1 titers of total mouse IgG (A), IgG1 (B), IgG2c (C), IgG2b (D), and IgG3 (E) were measured by ELISA. Filled diamonds, squares, and triangles represent each group (3 mice per group). Asterisks denote statistical significances (*P ≤ .05; **P ≤ .01).