Enhancement of p53 and p21 protein expression and inhibition of expression of cyclin A and D2 in erythroid cells cocultivated with HZ or treated with HNE. Erythroid cells were cocultivated with HZ (25μM HZ-heme) or treated with HNE (7μM) or kept as untreated controls (CTRL) in liquid culture. At indicated times cells were harvested, and 20 μg of lysate protein were analyzed for protein expression of p53 (A), p21 (B), cyclin A (C), and cyclin D2 (D). Proteins were separated by 1-dimensional (1D) SDS-PAGE and Western blotted. Transferred proteins were probed with specific primary antibodies and detected by binding of appropriate secondary antibodies. ECL-evidenced proteins of one representative experiment of 3. The corresponding densitometric values for each protein (arbitrary units) and the actin loading control are presented.