Figure 1
Figure 1. Methodology of in vitro assays. MNCs or CD34+ cells from each unit alone and in DCB combination were plated in methylcellulose and on MS5 stroma for CFC and CAFC assays, respectively. CFC assay was also performed after overnight incubation with thrombopoietin (TPO), kit ligand (KL), and Fms-like tyrosine kinase 3 ligand (Flt3L). CFC and CAFC were harvested from DCB cocultures for DNA extraction and quantitative PCR of short tandem repeats to establish donor origin. (Bottom left photograph) Composite of 4 images taken from the CFC experiments. Photomicrograph taken with Nikon Eclipse Ti-S microscope; objectives: 4× for CFC scoring, 10× and 20× for CAFC scoring. A Spot camera (Diagnostic Instruments) with Spot Version 4.0.2 software was used.

Methodology of in vitro assays. MNCs or CD34+ cells from each unit alone and in DCB combination were plated in methylcellulose and on MS5 stroma for CFC and CAFC assays, respectively. CFC assay was also performed after overnight incubation with thrombopoietin (TPO), kit ligand (KL), and Fms-like tyrosine kinase 3 ligand (Flt3L). CFC and CAFC were harvested from DCB cocultures for DNA extraction and quantitative PCR of short tandem repeats to establish donor origin. (Bottom left photograph) Composite of 4 images taken from the CFC experiments. Photomicrograph taken with Nikon Eclipse Ti-S microscope; objectives: 4× for CFC scoring, 10× and 20× for CAFC scoring. A Spot camera (Diagnostic Instruments) with Spot Version 4.0.2 software was used.

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