American Society of Hematology

Figure 4

$Figure 4. Cebpa/EYFP expression is found in a prominent fraction of early thymic progenitor cells with robust myeloid potential and thymic DCs. (A) Gating strategy and analysis of the percentage of EYFP+ cells in thymic progenitor compartments. (B) The percentage of EYFP+ cells (mean ± SD of 6 mice analyzed) among thymic cell subsets is highest in the DN1 fraction with a gradual decline toward more differentiated thymocyte populations. The corresponding percentages of Cebpa/EYFP+ cells of bone marrow LSK cells as well as of T and B cells of the peripheral blood of the same mice are also shown. (C) Analysis of the clonogenic myeloid potential of sorted EYFP+ and EYFP− DN1/DN2 cells incubated in Methocult 3231 supplemented with IL-3, SCF, GM-CSF, and G-CSF revealed robust myeloid potential among EYFP+ cells. Results show the mean ± SD of 3 independent experiments done in triplicate. **P < .01. (D) Analysis of the percentage of EYFP+ cells in plasmacytoid (CD11c+B220+) and conventional (CD11chiB220−) thymic DCs.$

Cebpa/EYFP expression is found in a prominent fraction of early thymic progenitor cells with robust myeloid potential and thymic DCs. (A) Gating strategy and analysis of the percentage of EYFP⁺ cells in thymic progenitor compartments. (B) The percentage of EYFP⁺ cells (mean ± SD of 6 mice analyzed) among thymic cell subsets is highest in the DN1 fraction with a gradual decline toward more differentiated thymocyte populations. The corresponding percentages of Cebpa/EYFP⁺ cells of bone marrow LSK cells as well as of T and B cells of the peripheral blood of the same mice are also shown. (C) Analysis of the clonogenic myeloid potential of sorted EYFP⁺ and EYFP⁻ DN1/DN2 cells incubated in Methocult 3231 supplemented with IL-3, SCF, GM-CSF, and G-CSF revealed robust myeloid potential among EYFP⁺ cells. Results show the mean ± SD of 3 independent experiments done in triplicate. **P < .01. (D) Analysis of the percentage of EYFP⁺ cells in plasmacytoid (CD11c⁺B220⁺) and conventional (CD11c^hiB220⁻) thymic DCs.

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