PGE₂-matured DCs show suppressed ability to attract naive CD4⁺ T cells. Negative isolated naive CD4⁺ T cells were allowed to migrate toward 24-hour culture supernatants from αDC1s and sDCs in transwell migration chambers (3 hours), in the absence (▭) or presence (▬) of CCR7-blocking antibody. The migrated T cells were collected from the bottom chamber and counted. The dotted line represents the average spontaneous migration of T cells in the absence of DC supernatants. Cumulative data (mean ± SEM) from 4 different donors.