Selectively elevated CCR7 expression on the surface of PGE₂-matured DCs occurs despite reduced levels of CCR7 mRNA and is suppressed by the exposure to CCL19. (A) Directly after harvesting from maturation cultures, αDC1s and sDCs were analyzed for the expression of surface CCR7 (left panel) and total (surface and intracellular; middle panel) CCR7 protein and for CCR7 mRNA expression (right panel). (B) Surface (top) and total (bottom) expression of CCR7 protein was analyzed in αDC1s, and sDCs matured in the absence or presence of exogenous CCL19 (100 ng/mL). Similar data were obtained in 3 independent experiments (3 different donors).