SAnxA1 inhibits PMN interaction with HUVECs. (A) Representative images of the flow chamber assay. Isolated PMNs were incubated with 10nM SAnxA1 for 10 minutes before flow over tumor necrosis factor-stimulated HUVEC monolayers. (B) Cumulative data for PMN capture, rolling, and adhesion after incubation with 10nM AnxA1 or SAnxA1 and flow over the HUVECs. (C) Concentration response for AnxA1 and SAnxA1 on the extent of PMN adhesion to HUVECs under flow. Data are mean ± SEM of 3 experiments performed with distinct donor cells. *P < .01 versus vehicle (as calculated on original values) and #P < .05 versus respective AnxA1 dataset.