Delayed and reduced recovery of Fak^−/− myeloid progenitors in response to 5-FU. (A) Whole BM cells from WT and Fak^−/− mice were harvested at 0, 3, 9, and 14 days after 5-FU injection and stained with FITC-conjugated anti–Mac-1, anti-B220, anti-CD3, anti–Gr-1, anti-F4/80, and anti-Ter119 antibodies. Subsequently, cells were stained with PE-conjugated antibody to Sca-1 and APC-conjugated–c-Kit antibody. c-Kit and Sca-1 expression were determined on lineage negative cells (LSK cells). Upper right quadrant in each dot blot indicates the percentage of LSK cells in WT and Fak^−/− mice at 0, 3, 9, and 14 days after 5-FU treatment. (B) Line chart represents the mean value of percent LSK cells in WT and Fak^−/− BM at the indicated time points after 5-FU treatment (n = 3-6 mice; *P < .05). (C) The percentage of Gr-1/Mac-1 double-positive cells in the BM of representative WT and Fak^−/− mouse is indicated in the upper right quadrant of each dot blot in response to 5-FU at indicated days after 5-FU treatment. (D) Line chart represents the percentage of Gr-1/Mac-1 double-positive cells in WT and Fak^−/− mice at 0, 3, 9, and 14 days after 5-FU treatment from 2 independent experiments (n = 3 mice per group, mean ± SEM; *P < .05).