Cre-mediated deletion of Fak in BM and spleen. (A) Cre-mediated deletion of FAK was induced by 3 intraperitoneal injections of 300 μg poly (I):(C) at 1-day intervals. One month after the final injection, DNA was extracted from BM and spleen and analyzed by PCR. Cre-mediated deletion of Fak was detected as a 327-bp fragment and that of WT Fak was observed as a 1.6-kb fragment. (B) Cre-mediated deletion of Fak after various times after poly (I):(C) treatment. One, 2, and 3 months following final injection of poly (I):(C), DNA was extracted from BM and analyzed by PCR. Cre-mediated deletion of Fak was detected as a 327-bp fragment and that of WT Fak was observed as a 1.6-kb fragment. Lanes 1, 2, and 3 represent WT FAK bands 1, 2, and 3 months after induction, respectively. Lanes 4, 5, and 6 represent Fak deletion in BM 1, 2, and 3 months after induction, respectively.